Molecular Biology and Real Time PCR Techniques

Course outline

What this programme covers, module by module.

1. 01Extracting nucleic acids and assessing their purity and concentration
2. 02Understanding primers, probes and reagents in the PCR reaction
3. 03Setting up conventional and real time PCR amplification workflows
4. 04Designing laboratory layouts that minimise amplicon cross contamination
5. 05Interpreting amplification curves, cycle thresholds and quantification data
6. 06Applying positive, negative and internal controls to validate assays
7. 07Troubleshooting failed reactions, inhibition and non specific amplification

What you will gain

• Extract and assess the quality of nucleic acids
• Set up conventional and real time PCR reactions
• Design workflows that minimise cross contamination
• Interpret amplification curves and quantification data
• Apply controls and validation to molecular assays
• Troubleshoot common PCR failures and inhibitors

Who should attend

• Molecular biologists and laboratory scientists
• Diagnostic laboratory technologists
• Research assistants using PCR methods
• Quality staff supporting molecular testing

Upcoming sessions

Fees are per delegate and exclude VAT. Public intakes are confirmed once minimum numbers are met. We also deliver this course on your own schedule, in-house or live online.