Skip to content
EasyUphillTRAININGCORPORATE DEVELOPMENT
HomeCoursesTrainersInsightsAboutContactBook a course+27 81 041 7673
Choose “Save as PDF” as the destination to email Molecular Biology and Real Time PCR Techniques to a client.
EasyUphill
CORPORATE DEVELOPMENT
+27 81 041 7673
training@eubiz.co.za
68 Sunstone Street, Germiston, 1401
eubiz.co.za
Laboratory & Microbiology

Molecular Biology and Real Time PCR Techniques

Duration
5 Days
Delivery
In-house, public or online
From
R18,000 per delegate
Certificate
On completion
Course overview

This practical course introduces the principles and applications of molecular biology with a focus on the polymerase chain reaction. Delegates learn nucleic acid extraction, primer design considerations and both conventional and real time PCR workflows. The programme also covers contamination control and result interpretation for diagnostic and research settings.

What you will be able to do
  • Extract and assess the quality of nucleic acids
  • Set up conventional and real time PCR reactions
  • Design workflows that minimise cross contamination
  • Interpret amplification curves and quantification data
  • Apply controls and validation to molecular assays
  • Troubleshoot common PCR failures and inhibitors
Who should attend
  • Molecular biologists and laboratory scientists
  • Diagnostic laboratory technologists
  • Research assistants using PCR methods
  • Quality staff supporting molecular testing
Course outline
01
Extracting nucleic acids and assessing their purity and concentration
02
Understanding primers, probes and reagents in the PCR reaction
03
Setting up conventional and real time PCR amplification workflows
04
Designing laboratory layouts that minimise amplicon cross contamination
05
Interpreting amplification curves, cycle thresholds and quantification data
06
Applying positive, negative and internal controls to validate assays
07
Troubleshooting failed reactions, inhibition and non specific amplification
Upcoming public sessions
DatesVenueDurationFee per delegate
2 to 6 Nov 2026Pretoria5 DaysR18,000
16 to 20 Nov 2026Durban5 DaysR18,000
30 Nov to 4 Dec 2026Cape Town5 DaysR18,000
11 to 15 Jan 2027Johannesburg5 DaysR18,000
22 to 26 Feb 2027London5 DaysUS$1,800
26 to 30 Apr 2027Dubai5 DaysUS$1,800
5 to 9 Jul 2027Pretoria5 DaysR18,000
Fees exclude VAT. This course can also be delivered in-house at your premises on dates that suit your team, or live online.
To book or ask for an in-house quote

Reply to the email this brochure came with, call +27 81 041 7673, or visit eubiz.co.za and open this course.

EasyUphill Training & ConsultingTailored training that moves teams forward.
+27 81 041 7673  ·  training@eubiz.co.za
68 Sunstone Street, Germiston, 1401  ·  eubiz.co.za